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Electrospray ionization with ambient pressure ion mobility separation and mass analysis by orthogonal time‐of‐flight mass spectrometry
Author(s) -
Steiner Wes E.,
Clowers Brian H.,
Fuhrer Katrin,
Gonin Marc,
Matz Laura M.,
Siems William F.,
Schultz Albert J.,
Hill Herbert H.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.495
Subject(s) - chemistry , ion mobility spectrometry , mass spectrometry , analytical chemistry (journal) , ion , electrospray , fragmentation (computing) , electrospray ionization , dissociation (chemistry) , collision induced dissociation , ionization , ion source , ambient ionization , time of flight mass spectrometry , extractive electrospray ionization , desorption electrospray ionization , mass spectrum , chromatography , chemical ionization , tandem mass spectrometry , sample preparation in mass spectrometry , organic chemistry , computer science , operating system
Rapid screening and identification of drug and other mixtures are possible using a novel ambient pressure high‐resolution ion mobility (APIMS) orthogonal reflector time‐of‐flight mass spectrometer (TOFMS). Departing ions from the APIMS drift tube traversed a pressure interface between the APIMS and TOFMS where they were subjected to numerous gas collisions that could produce selective fragmentation. By increasing the accelerating field in the pressure interface region, the ions generated using water‐cooled electrospray ionization (ESI) underwent collision‐induced dissociation (CID). Mixtures of ESI ions were separated by APIMS based on their respective size‐to‐charge ( s/z ) ratios while CID and analysis of mass‐to‐charge ( m/z ) ratios occurred in the pressure interface and TOFMS. Product ions that were formed in this pressure interface region could be readily assigned to precursor ions by matching the mobility drift times. This process was demonstrated by the examination of a mixture of amphetamines and the resulting fragmentation patterns of the mobility‐separated precursor ion species [M + H] + . Copyright © 2001 John Wiley & Sons, Ltd.