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Detergent enhancement of on‐tissue protein analysis by matrix‐assisted laser desorption/ionization imaging mass spectrometry
Author(s) -
Mainini Veronica,
Angel Peggi M.,
Magni Fulvio,
Caprioli Richard M.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4850
Subject(s) - chemistry , maldi imaging , mass spectrometry , mass spectrometry imaging , desorption , matrix assisted laser desorption/ionization , analyte , chromatography , sodium dodecyl sulfate , matrix (chemical analysis) , ionization , analytical chemistry (journal) , ion , organic chemistry , adsorption
Matrix‐Assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS) is a molecular technology that allows simultaneous investigation of the content and spatial distribution of molecules within tissue. In this work, we examine different classes of detergents, the anionic sodium dodecyl sulfate (SDS), the nonionic detergents Triton X‐100, Tween 20 and Tween 80, and the zwitterionic 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS) for use in MALDI IMS of analytes above m/z 4000. These detergents were found to be compatible with MALDI MS and did not cause signal suppression relative to non‐detergent applications and did not produce interfering background signals. In general, these detergents enhanced signal acquisition within the mass range m/z 4–40 000. Adding detergents into the matrix was comparable with the separate application of detergent and matrix. Evaluation of spectra collected from organ‐specific regions of a whole mouse pup section showed that different detergents perform optimally with different organs, indicating that detergent selection should be optimized on the specific tissue for maximum gain. These data show the utility of detergents towards enhancement of protein signals for on‐tissue MALDI IMS analysis. Copyright © 2010 John Wiley & Sons, Ltd.