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Cavitation of liquid flow in electrospray mass spectrometry especially for addition of internal calibrant in ‘accurate mass’ measurements
Author(s) -
Lord Gwyn A.,
Lim ChangKee
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4828
Subject(s) - chemistry , mass spectrometry , analyte , chromatography , analytical chemistry (journal) , electrospray , liquid chromatography–mass spectrometry
‘Accurate mass’ measurements in electrospray mass spectrometry are becoming more prevalent with the increasing availability of mass spectrometers with sufficient resolution. A reference compound is ideally admitted separately but almost simultaneously with the analyte and this is achieved by use of a ‘dual sprayer’ or voltage switching between reference and sample sprayers. This paper describes a novel third method, relying on cavitation (segmentation) of the liquid stream containing the reference compound, allowing the sample to ionise independently from the sample, thus preventing interference. The technique may also find application in kinetic experiments, such as protein folding studies. A high‐performance liquid chromatography (HPLC) injector was also used to provide a reference compound, producing Gaussian‐shaped profiles of varying ion intensity, thus allowing easier selection of a desirable measurement point where the intensities of the reference and analyte were similar. Copyright © 2010 John Wiley & Sons, Ltd.