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Study of plasma metabolic profiling and biomarkers of chronic unpredictable mild stress rats based on gas chromatography/mass spectrometry
Author(s) -
Li ZhenYu,
Zheng XingYu,
Gao XiaoXia,
Zhou YuZhi,
Sun HaiFeng,
Zhang LiZeng,
Guo XiaoQing,
Du GuanHua,
Qin XueMei
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4809
Subject(s) - chemistry , metabolomics , chromatography , gas chromatography–mass spectrometry , mass spectrometry , metabolome , metabolism , amino acid , metabolic pathway , biochemistry
A metabolomic investigation of chronic unpredictable mild stress (CUMS) rats was carried out. Plasma obtained from Sprague‐Dawley (SD) rats treated by CUMS was analyzed using gas chromatography/mass spectrometry. Thirty‐seven metabolites were identified among the detected compounds. Subsequent data analysis using the t test and principal component analysis (PCA) revealed significant metabolic changes in the rats' plasma after CUMS treatment. Clear separation between the model and control group was achieved, and the level of twelve metabolites, including amino acids, sugar, organic acids and fatty acids, were significantly different between plasma samples from the controls and CUMS group. These observations suggested that the depressed state may be associated with perturbation of amino acid metabolism, energy metabolism and glycometabolism. The study suggested that the metabolomics approach could be used as a potential powerful tool to investigate the biochemical change in certain physiopathological conditions, such as depression, as an early diagnostic means. Copyright © 2010 John Wiley & Sons, Ltd.