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Rapid screening and characterization of metabolites from a marine‐derived actinomycete by high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry
Author(s) -
Liu ChengWei,
Lu YuanYuan,
Yang ZhenZheng,
Xing YingYing,
Xi Tao
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4744
Subject(s) - chemistry , electrospray ionization , mass spectrometry , quadrupole time of flight , chromatography , high performance liquid chromatography , ion , quadrupole , ionization , peptide , electrospray , dissociation (chemistry) , organic chemistry , biochemistry , physics , atomic physics
A rapid and reliable method has been optimized and established for the analysis of the metabolites from a marine actinomycete by high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry (HPLC/QTOF MS/MS). From MS/MS spectra, the product ions of [M + H] + were recorded to provide abundant structural information of the mother nucleus and peptide moieties. Using the QTOF MS/MS and in‐source collision‐induced dissociation (in‐source CID) techniques, three main metabolites including actinomycin D, actinomycin V and actinomycin I were determined and characterized by elemental compositions of precursor and product ions (<7 ppm). Additionally, this method provided information about the compositions of the peptide residues and the sequences of the amino acid from a series of fragment ions. It proved useful for the identication of the metabolites in marine samples which have similar structures especially when there were no reference compounds available. Copyright © 2010 John Wiley & Sons, Ltd.