Premium
Targeted serum metabolite profiling of nucleosides in esophageal adenocarcinoma
Author(s) -
Djukovic Danijel,
Baniasadi Hamid R.,
Kc Ravi,
Hammoud Zane,
Raftery Daniel
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4739
Subject(s) - chemistry , uridine , metabolite , cytidine , high performance liquid chromatography , adenocarcinoma , cancer , biochemistry , rna , chromatography , medicine , gene , enzyme
Abstract Nucleosides are indicators of the whole‐body turnover of transfer RNA. Based on the activity of cancer cells these molecules could potentially be used as cancer biomarkers, and several studies have determined that the metabolic levels of nucleosides are significantly altered in cancer patients compared to control groups. Here we report a targeted metabolite investigation of serum nucleosides in esophageal adenocarcinoma specimens. We quantified eight nucleosides using high‐performance liquid chromatography/triple quadrupole mass spectrometry (HPLC/TQMS) and determined that the metabolic levels of 1‐methyladenosine ( p <2.14 × 10 −7 ), N 2 ,N 2 ‐dimethylguanosine ( p <2.78 × 10 −7 ), N 2 ‐ methylguanosine ( p <2.48 × 10 −6 ) and cytidine ( p <6.98 × 10 −4 ) were significantly elevated while the concentration of uridine ( p <3.74 × 10 −3 ) was significantly lowered in serum samples from cancer patients compared to those of control group. Our results suggest that nucleosides could potentially serve as useful biomarkers to identify esophageal adenocarcinoma. Copyright © 2010 John Wiley & Sons, Ltd.