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Ionspray microchip
Author(s) -
Pól Jaroslav,
Kauppila Tiina J.,
Franssila Sami,
Kotiaho Tapio,
Kostiainen Risto
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4678
Subject(s) - chemistry , nebulizer , thermospray , analytical chemistry (journal) , atmospheric pressure , chromatography , atmospheric pressure chemical ionization , mass spectrometry , ion source , analyte , photoionization , volumetric flow rate , ionization , chemical ionization , ion , tandem mass spectrometry , selected reaction monitoring , medicine , oceanography , physics , organic chemistry , quantum mechanics , anesthesia , geology
An ionspray microchip is introduced. The chip is based on the earlier presented nebulizer microchip that consists of glass and silicon plates bonded together. A liquid inlet channel, nebulizer gas inlet, and nozzle are etched on the silicon plate and a platinum heater is integrated on the glass plate. The nebulizer microchip has been previously used in atmospheric pressure chemical ionization, atmospheric pressure photoionization, sonic spray ionization, and thermospray ionization modes. In this work we show that the microchip can be operated also in ionspray mode by introducing high voltage to the silicon plate of the microchip. The effects of operation parameters (voltage, nebulizer gas pressure, sample solution flow rate, solvent composition, and analyte concentration) on the performance of the ion spray microchip were studied. Under optimized conditions the microchip provides efficient ionization of small and large compounds and good quantitative performance. The feasibility of the ion spray microchip in liquid chromatography/mass spectrometry (LC/MS) was demonstrated by the analysis of tryptic peptides of bovine serum albumin. Copyright © 2010 John Wiley & Sons, Ltd.