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Simplified capillary electrophoresis nanospray sheath‐flow interface for high efficiency and sensitive peptide analysis
Author(s) -
Wojcik Roza,
Dada Oluwatosin O.,
Sadilek Martin,
Dovichi Norman J.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4672
Subject(s) - chemistry , capillary electrophoresis , analytical chemistry (journal) , capillary action , common emitter , electrospray , chromatography , mass spectrometry , electrophoresis , detection limit , electrokinetic phenomena , micellar electrokinetic chromatography , optoelectronics , materials science , composite material
We report a simple nanospray sheath‐flow interface for capillary electrophoresis. This interface relies on electrokinetic flow to drive both the separation and the electrospray; no mechanical pump is used for the sheath flow. This system was interfaced with an LCQ mass spectrometer. The best results were observed with a 2‐µm diameter emitter tip and a 1‐mm spacing between the separation capillary tip and the emitter tip. Under these conditions, mass detection limits (3 σ ) of 100 amol were obtained for insulin receptor fragment 1142‐1153. The separation efficiency exceeded 200,000 plates for this compound. The relative standard deviation generated during continual infusion of a 50 µM solution of angiotensin II was 2% for the total ion count and 3% for the extracted ion count over a 40‐min period. Finally, the interface was also demonstrated for negative ion mode. Copyright © 2010 John Wiley & Sons, Ltd.