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Rapid screening and identification of caffeic acid and its esters in Erigeron breviscapus by ultra‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Liao ShangGao,
Zhang LiJuan,
Li CuiBing,
Lan YanYu,
Wang AiMin,
Huang Yong,
Zhen Lin,
Fu XiaoZhong,
Zhou Wen,
Qi XiaoLan,
Guan ZhiZhong,
Wang YongLin
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4662
Subject(s) - chemistry , chromatography , caffeic acid , tandem mass spectrometry , tandem , mass spectrometry , liquid chromatography–mass spectrometry , organic chemistry , antioxidant , materials science , composite material
Caffeic acid and its esters (CAEs) are widely distributed in the plant kingdom and have been reported to elicit a wide range of exceptional biological activities. Present methods for screening and characterization of CAEs normally need the use of liquid chromatography diode‐array detection/multistage mass spectrometry (LC‐DAD/MS n ). In this report, a rapid and efficient method coupling ultra‐performance liquid chromatography (UPLC) with fragment‐targeted multi‐reaction monitoring (MRM) has been developed for screening CAEs in a crude extract of Erigeron breviscapus , while a UPLC/quasi‐MS n method has been applied in the structural identification of these compounds. Furthermore, a simple quasi‐UPLC/MS/MS method based on in‐source collision‐induced dissociation (CID) has been proposed for rapid identification of the CAEs. As a result, a total of more than 34 CAEs were detected and their structures characterized. Nine of them were reported from E. breviscapus for the first time. Applications of these strategies in the chemical investigation of an injection of E. breviscapus resulted in the identifications of 16 CAEs. These strategies, if appropriate modifications are made, will be very useful in screening and characterization not only of CAEs, but of other structural types of compounds in various complex matrices. Copyright © 2010 John Wiley & Sons, Ltd.