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A rapid and specific derivatization procedure to identify acyl‐glucuronides by mass spectrometry
Author(s) -
Vaz Alfin D. N.,
Wang Wei Wei,
Bessire Andrew J.,
Sharma Raman,
Hagen Anne E.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4621
Subject(s) - chemistry , derivatization , chromatography , hydroxylamine , hydroxamic acid , mass spectrometry , organic chemistry , alkyl , derivative (finance) , financial economics , economics
A simple procedure is described to identify acyl‐glucuronides by coupled liquid chromatography/mass spectrometry after derivatization to a hydroxamic acid with hydroxylamine. The reaction specificity obviates the need for isolation of the acyl‐glucuronide from an extract. Glucuronides derived from carbamic acids, and alkyl‐ and aromatic amines, are inert to the derivatization reaction conditions, making the hydroxamic acid derivative a fingerprint for acyl‐glucuronides. Copyright © 2010 John Wiley & Sons, Ltd.