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Thin‐layer chromatography and mass spectrometry coupled using proximal probe thermal desorption with electrospray or atmospheric pressure chemical ionization
Author(s) -
Ovchinnikova Olga S.,
Van Berkel Gary J.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4551
Subject(s) - chemistry , desorption electrospray ionization , ambient ionization , analytical chemistry (journal) , atmospheric pressure , mass spectrometry , ionization , extractive electrospray ionization , chemical ionization , direct electron ionization liquid chromatography–mass spectrometry interface , electrospray ionization , dart ion source , thermal ionization , matrix assisted laser desorption electrospray ionization , ion source , electrospray , atmospheric pressure chemical ionization , chromatography , ion , thermal ionization mass spectrometry , sample preparation in mass spectrometry , electron ionization , oceanography , organic chemistry , geology
An atmospheric pressure proximal probe thermal desorption sampling method coupled with secondary ionization by electrospray or atmospheric pressure chemical ionization was demonstrated for the mass spectrometric analysis of a diverse set of compounds (dyestuffs, pharmaceuticals, explosives and pesticides) separated on various high‐performance thin‐layer chromatography plates. Line scans along or through development lanes on the plates were carried out by moving the plate relative to a stationary heated probe positioned close to or just touching the stationary phase surface. Vapors of the compounds thermally desorbed from the surface were drawn into the ionization region of a combined electrospray ionization/atmospheric pressure chemical ionization source where they merged with reagent ions and/or charged droplets from a corona discharge or an electrospray emitter and were ionized. The ionized components were then drawn through the atmospheric pressure sampling orifice into the vacuum region of a triple quadrupole mass spectrometer and detected using full scan, single ion monitoring, or selected reaction monitoring mode. Studies of variable parameters and performance metrics including the proximal probe temperature, gas flow rate into the ionization region, surface scan speed, read‐out resolution, detection limits, and surface type are discussed. Published in 2010 by John Wiley & Sons, Ltd.