Premium
Minimizing analyte electrolysis in electrospray ionization mass spectrometry using a redox buffer coated emitter electrode
Author(s) -
PeintlerKrivan Emese,
Van Berkel Gary J.,
Kertesz Vilmos
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4522
Subject(s) - chemistry , analyte , extractive electrospray ionization , electrospray ionization , mass spectrometry , electrolysis , direct electron ionization liquid chromatography–mass spectrometry interface , analytical chemistry (journal) , common emitter , electrode , redox , buffer (optical fiber) , ionization , electrospray , sample preparation in mass spectrometry , chromatography , chemical ionization , inorganic chemistry , optoelectronics , ion , organic chemistry , electrolyte , telecommunications , physics , computer science
An emitter electrode with an electroactive poly(pyrrole) (PPy) polymer film coating was constructed for use in electrospray ionization mass spectrometry (ESI‐MS). The PPy film acted as a surface‐attached redox buffer limiting the interfacial potential of the emitter electrode. While extensive oxidation of selected analytes (reserpine and amodiaquine) was observed in positive ion mode ESI using a bare metal (gold) emitter electrode, the oxidation was suppressed for these same analytes when using the PPy‐coated electrode. A semi‐quantitative relationship between the rate of oxidation observed and the interfacial potential of the emitter electrode was shown. The redox buffer capacity, and therefore the lifetime of the redox buffering effect, correlated with the oxidation potential of the analyte and with the magnitude of the film charge capacity. Online reduction of the PPy polymer layer using negative ion mode ESI between analyte injections was shown to successfully restore the redox buffering capacity of the polymer film to its initial state. Published in 2010 by John Wiley & Sons, Ltd.