z-logo
Premium
Rapid characterization of high/low producer CHO cells using matrix‐assisted laser desorption/ionization time‐of‐flight
Author(s) -
Feng Huatao,
Wong Niki S. C.,
Sim Lyn Chiin,
Wati Lydia,
Ho Yingswan,
Lee May May
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4506
Subject(s) - chemistry , chinese hamster ovary cell , mass spectrometry , desorption , chromatography , cell culture , matrix (chemical analysis) , analytical chemistry (journal) , matrix assisted laser desorption/ionization , recombinant dna , mass spectrum , bioprocess , ionization , cell , biochemistry , ion , chemical engineering , adsorption , receptor , organic chemistry , biology , gene , engineering , genetics
An intact‐cell mass spectrometry (ICM) method using matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) was evaluated for the screening of stable recombinant Chinese hamster ovary (CHO) cell lines, an important mammalian cell line in bioprocessing. With rapid and simple cell pretreatments, viabilities of cells could be rapidly distinguished on the different fingerprints of mass spectra. Detectable m/z values on cell surfaces and their relative intensities were processed by two biostatistical methods, principle components analysis (PCA) and partial least squares (PLS), with promising results. Discrimination among cell lines with different expressed recombinant proteins or different productivities could be achieved. The ICM method has the advantage of providing multiple parameters simultaneously and possesses the potential to become a powerful method for routine monitoring of bioprocesses. Copyright © 2010 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here