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In situ probing of cholesterol in astrocytes at the single‐cell level using laser desorption ionization mass spectrometric imaging with colloidal silver
Author(s) -
Perdian D. C.,
Cha Sangwon,
Oh Jisun,
Sakaguchi Donald S.,
Yeung Edward S.,
Lee Young Jin
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4491
Subject(s) - chemistry , desorption , mass spectrometry , adduct , in situ , ionization , ion , analytical chemistry (journal) , mass spectrometry imaging , laser , membrane , chromatography , biochemistry , adsorption , optics , organic chemistry , physics
Mass spectrometric imaging has been utilized to localize individual astrocytes and to obtain cholesterol populations at the single‐cell level in laser desorption ionization (LDI) with colloidal silver. The silver ion adduct of membrane‐bound cholesterol was monitored to detect individual cells. Good correlation between mass spectrometric and optical images at different cell densities indicates the ability to perform single‐cell studies of cholesterol abundance. The feasibility of quantification is confirmed by the agreement between the LDI‐MS ion signals and the results from a traditional enzymatic fluorometric assay. We propose that this approach could be an effective tool to study chemical populations at the cellular level. Published in 2010 by John Wiley & Sons, Ltd.