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Combination of different liquid chromatography/mass spectrometry technologies for the identification of transformation products of rhodamine B in groundwater
Author(s) -
Müller Alexander,
Weiss Stefan C.,
Schulz Wolfgang,
Seitz Wolfram,
Albert Roger,
Ruck Wolfgang K. L.,
Weber Walter H.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4430
Subject(s) - chemistry , chromatography , mass spectrometry , rhodamine b , high performance liquid chromatography , rhodamine , tandem mass spectrometry , liquid chromatography–mass spectrometry , quadrupole time of flight , fluorescence , biochemistry , physics , photocatalysis , quantum mechanics , catalysis
Rhodamine B and its five de‐ethylated transformation products could be identified in a groundwater sample. Using high‐performance thin‐layer chromatography (HPTLC) six fluorescent zones were detected in the sample. In order to identify the compounds in the zones by exact mass mass spectrometry (MS) measurements and tandem mass spectrometry (MS/MS), they were extracted from the HPTLC plate for subsequent analysis by nano‐chip high‐performance liquid chromatography quadrupole‐time‐of‐flight mass spectrometry (nano‐chip HPLC/QTOFMS). In addition, chemical derivatisation experiments on HPTLC plates were applied to detect the presence of a primary amino group in the transformation products. From the combined analytical results it was possible to allocate rhodamine B and its five de‐ethylated transformation products to the six different HPTLC zones. The quantification of rhodamine B in different groundwater samples was carried out by a high‐performance liquid chromatography/triple quadrupole mass spectrometry (HPLC/MS/MS). The maximum detected concentration of rhodamine B was 83 µg L −1 . Copyright © 2010 John Wiley & Sons, Ltd.