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Liquid chromatography/mass spectrometry analysis revealing preferential occurrence of non‐arachidonate‐containing phosphatidylinositol bisphosphate species in nuclei and changes in their levels during cell cycle
Author(s) -
Ogiso Hideo,
Nakamura Kazuhiro,
Yatomi Yutaka,
Shimizu Takao,
Taguchi Ryo
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4415
Subject(s) - chemistry , phosphatidylinositol , nucleus , hela , cell , mass spectrometry , biochemistry , chromatography , microbiology and biotechnology , signal transduction , biology
Phosphatidylinositol phosphates (PtdIns Ps ) are present within the nucleus, as well as in the membrane. In this mass spectrometry study, different acyl‐containing species of endonuclear PtdIns Ps were analyzed in order to clearly understand the role of individual molecular species. A (34:1) acyl‐containing phosphatidylinositol bisphosphate [PtdIns P 2 (34:1)] and PtdIns P 2 (36:1) were preferentially detected in envelope‐less nuclei prepared from various cultured human cells, while PtdIns P 2 (38:4) was not a major component within these nuclei. A significant amount of PtdIns P 2 (34:0) was detected in the HeLa cell nucleus, but not in the A431 and THP‐1 cell nuclei. During the cell cycle in HeLa cells, PtdIns P 2 (34:0) levels increased in the early G1 phase, and then gradually decreased through S phase, while PtdIns P 2 (34:1) levels tended to decrease only in late G1 phase and PtdIns P 2 (38:4) did not change significantly. Thus, individual PtdIns P 2 species apparently play different roles in nuclear events based on individual regulation of endonuclear levels. The non‐arachidonate‐containing species were also detected in normal human blood and fluids, suggesting that these minor species may have unique functions in the human body. The techniques used in this study will be applied to clinical studies on a PtdIns Ps metabolism. Copyright © 2010 John Wiley & Sons, Ltd.