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Atmospheric pressure laser desorption/ionization of plant metabolites and plant tissue using colloidal graphite
Author(s) -
Perdian D. C.,
Schieffer G. M.,
Houk R. S.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4405
Subject(s) - chemistry , desorption , mass spectrometry , graphite , chromatography , matrix (chemical analysis) , colloid , ionization , ion , analytical chemistry (journal) , organic chemistry , adsorption
Abstract Colloidal graphite is a promising matrix for atmospheric pressure laser desorption/ionization mass spectrometry. Intact [M+H] + and [M–H] − ions are readily produced from a wide range of small molecule plant metabolites, particularly anthocyanins, fatty acids, lipids, glycerides, and ceramides. Compared with a more traditional organic acid matrix, colloidal graphite provides more efficient ionization for small hydrophobic molecules and has a much cleaner background spectrum, especially in negative ion mode. Some important metabolites, e.g., fatty acids and glycosylated flavonoids, can be observed from Arabidopsis thaliana leaf and flower petal tissues in situ . Copyright © 2010 John Wiley & Sons, Ltd.