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The effects of differentiated water supply after anthesis and nitrogen fertilization on δ 15 N of wheat grain
Author(s) -
Raimanová Ivana,
Haberle Jan
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4382
Subject(s) - nitrogen , anthesis , delta , human fertilization , chemistry , agronomy , fertilizer , crop , wheat grain , soil water , field experiment , zoology , environmental science , biology , soil science , cultivar , organic chemistry , engineering , aerospace engineering
The δ 15 N signature of plants integrates various processes in soil and plant. In this study, the effect of different water regimes applied during the period of grain growth of winter wheat on grain δ 15 N was examined in a 4‐year field experiment. The treatments comprised water shortage (S), an ample water supply (W), and rain‐fed crop (R). Zero fertilization (N0) and 200 kg N.ha −1 in mineral fertilizer (N1) treatments were studied. The grain 15 N was determined during grain growth and at maturity. The water regime, nitrogen application and year had a significant effect on mature grain δ 15 N ( p < 0.001). Water and nitrogen explained 54.6% of the variability of δ 15 N in the experiment, the year accounted for 10.7% and the interactions for another 19.6% of the total variability. The analysis of non‐mature grain δ 15 N showed significant effects of N and year but not of water. Nitrogen fertilization reduced the δ 15 N of mature grain in years by 0.7–6.3‰ in comparison with N0 plants; the reduction was more pronounced under stress (average reduction by 4.1‰) than under rain‐fed (2.4‰) and ample water supply (2.2‰). Water stress decreased the grain δ 15 N in fertilized wheat, by 0.1–2.1‰ and 0.6–3.6‰ in experimental years, on average by 1.30‰ and 1.79‰ in comparison with the R and W water supply, respectively. The effect of water supply was not significant in non‐fertilized wheat. A significant negative linear relationship between grain N concentration and δ 15 N in maturity or during the grain growth (R 2 = 0.83, R 2 = 0.76, respectively) was found. The observed sources of grain δ 15 N variability should be taken into consideration when analyzing and interpreting the data on the δ 15 N signature of plant material from field conditions. Copyright © 2010 John Wiley & Sons, Ltd.