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Applications of stable isotope ratio mass spectrometry in cattle dung carbon cycling studies
Author(s) -
Dungait Jennifer A. J.,
Bol Roland,
LopezCapel Elisa,
Bull Ian D.,
Chadwick David,
Amelung Wulf,
Granger Steven J.,
Manning David A. C.,
Evershed Richard P.
Publication year - 2010
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4332
Subject(s) - chemistry , isotope ratio mass spectrometry , soil water , environmental chemistry , organic matter , soil organic matter , stable isotope ratio , isotopes of carbon , carbon fibers , isotope analysis , soil carbon , cycling , mass spectrometry , total organic carbon , soil science , ecology , environmental science , chromatography , organic chemistry , physics , materials science , quantum mechanics , biology , composite number , composite material , history , archaeology
Understanding the fate of dung carbon (C) in soils is challenging due to the ubiquitous presence of the plant‐derived organic matter (OM), the source material from which both dung‐derived OM and soil organic matter (SOM) predominantly originate. A better understanding of the fate of specific components of this substantial source of OM, and thereby its contribution to C cycling in terrestrial ecosystems, can only be achieved through the use of labelled dung treatments. In this short review, we consider analytical approaches using bulk and compound‐specific stable carbon isotope analysis that have been utilised to explore the fate of dung‐derived C in soils. Bulk stable carbon isotope analyses are now used routinely to explore OM matter cycling in soils, and have shown that up to 20% of applied dung C may be incorporated into the surface soil horizons several weeks after application, with up to 8% remaining in the soil profile after one year. However, whole soil δ 13 C values represent the average of a wide range of organic components with varying δ 13 C values and mean residence times in soils. Several stable 13 C isotope ratio mass spectrometric methods have been developed to qualify and quantify different fractions of OM in soils and other complex matrices. In particular, thermogravimetry‐differential scanning calorimetry‐isotope ratio mass spectrometry (TG‐DSC‐IRMS) and gas chromatography‐combustion‐IRMS (GC‐C‐IRMS) analyses have been applied to determine the incorporation and turnover of polymeric plant cell wall materials from C 4 dung into C 3 grassland soils using natural abundance 13 C isotope labelling. Both approaches showed that fluxes of C derived from polysaccharides, i.e. as cellulose or monosaccharide components, were more similar to the behaviour of bulk dung C in soil than lignin. However, lignin and its 4‐hydroxypropanoid monomers were unexpectedly dynamic in soil. These findings provide further evidence for emerging themes in biogeochemical investigations of soil OM dynamics that challenge perceived concepts of recalcitrance of C pools in soils, which may have profound implications for the assessment of the potential of agricultural soils to influence terrestrial C sinks. Copyright © 2010 John Wiley & Sons, Ltd.