Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high‐resolution mass spectrometry | Zendy

Xiong Aizhen | Zendy; Yang Li | Zendy; He Yuqi | Zendy; Zhang Fang | Zendy; Wang Jun | Zendy; Han Han | Zendy; Wang Changhong | Zendy; Bligh S. W. Annie | Zendy; Wang Zhengtao | Zendy

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Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high‐resolution mass spectrometry

Author(s) -

Xiong Aizhen,

Yang Li,

He Yuqi,

Zhang Fang,

Wang Jun,

Han Han,

Wang Changhong,

Bligh S. W. Annie,

Wang Zhengtao

Publication year - 2009

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.4329

Subject(s) - chemistry , chromatography , pyrrolizidine , tandem mass spectrometry , mass spectrometry , electrospray ionization , pyrrolizidine alkaloid , electrospray , glucuronidation , alkaloid , metabolite , liquid chromatography–mass spectrometry , biochemistry , stereochemistry , enzyme , microsome

Hepatotoxic pyrrolizidine alkaloid (HPA)‐containing plants have always been a threat to human and livestock health worldwide. Adonifoline, a main HPA in Senecio scandens Buch.‐Ham. ex D. Don (Qianli guang), was used officially as an infusion in cases of oral and pharyngeal infections in China. In this study i n vivo metabolism of adonifoline was studied for the first time by identifying the metabolites of adonifoline present in bile, urine and feces of rats using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS n ) (ion trap) as well as liquid chromatography/electrospray ionization high‐resolution mass spectrometry (LC/ESI‐HRMS) (quadrupole‐time of flight). In total 19 metabolites were identified and, among them, retronecine‐ N ‐oxides were confirmed by matching their fragmentation patterns with their fully characterized synthetic compounds. These metabolites are all involved in both phase I and phase II metabolic processes and the principal in vivo metabolism pathways of adonifoline were proposed. Copyright © 2009 John Wiley & Sons, Ltd.

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