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Detection of pathogenic Verticillium spp. using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Tao Jie,
Zhang Guiming,
Jiang Zide,
Cheng Yinghui,
Feng Jianjun,
Chen Zhinan
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4296
Subject(s) - chemistry , mass spectrometry , time of flight mass spectrometry , chromatography , matrix (chemical analysis) , ionization , analytical chemistry (journal) , ion , organic chemistry
Verticillium spp. have been listed by the European and Mediterranean Plant Protection Organization (EPPO) and China as plant quarantine pests. Although attempts have been made to develop a simple routine laboratory assay to detect these organisms, none are routinely used. We describe for the first time a robust assay for reliable identification of Verticillium spp. using protein fingerprinting data obtained by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry(MALDI‐TOF‐MS). Several sample preparation methods and matrices were investigated to improve mass spectra for the routine identification of six species of Verticillium spp.( Verticillium dahiliae , V. alboatrum , V. fungicola , V. nigrescens , and V. lecanii ) by MALDI‐TOF‐MS. Using the optimized experimental method, we constructed a protein fingerprint database for six species of Verticillium and established a analysis criteria of log(Score). This MALDI‐TOF‐MS protocol should prove useful as a rapid and reliable assay for distinguishing different Verticillium spp. Copyright © 2009 John Wiley & Sons, Ltd.