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Rapid simultaneous quantitative determination of different small pharmaceutical drugs using a conventional matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry system
Author(s) -
Persike Markus,
Karas Michael
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4283
Subject(s) - chemistry , chromatography , mass spectrometry , analytical chemistry (journal) , linearity , accuracy and precision , matrix (chemical analysis) , resolution (logic) , ionization , sample preparation , standard deviation , relative standard deviation , detection limit , ion , statistics , physics , mathematics , organic chemistry , quantum mechanics , artificial intelligence , computer science
The present study establishes a simple, rapid and sensitive method for the simultaneous quantification of different small pharmaceutical drugs using a matrix‐assisted laser desorption/ionization source (MALDI) coupled with a time‐of‐flight (TOF) mass analyzer. Neither time‐consuming sample preparation, nor special target plates, isotopically labelled internal standards or other extra equipment are necessary. A simple standard dried‐droplet preparation with the common matrix α ‐cyano‐4‐hydroxycinnamic acid (CHCA) was used. The background signals of CHCA in the low‐mass region did not pose the presumed problem, because the sensitivity, resolution and mass accuracy of a modern MALDI‐TOF MS system is sufficient to overcome this difficulty. Four experiments were performed in order to verify the quantification method. First, ten different phenothiazines were quantified in the range of 5–2000 nM (1–880 ng/mL). A good precision (relative standard deviation (RSD) 4.4–9.3%), linearity (R 2 >0.99) and accuracy (error 4.7–11%) was obtained in all cases. Additionally, simultaneous quantification of these ten phenothiazines was carried out in human plasma without prior chromatographic separation in the range of 2–1750 ng/mL yielding good linearity, precision and accuracy (mean RSD 7.6%; R 2 >0.99, mean error 8.0%). Accordingly, a quantitative analysis of ten chemically and pharmaceutically unrelated drugs was performed in the same way. A comparable linearity (R 2 >0.99), precision (mean RSD 7.6%) and accuracy (mean error 8.3%) was obtained in the range of 5–2000 nM. Finally, the prazosin content of a commercial tablet was directly determined without further purification steps. Copyright © 2009 John Wiley & Sons, Ltd.