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Analysis of urinary nucleosides. II. Comparison of mass spectrometric methods for the analysis of urinary nucleosides
Author(s) -
Dudley Edward,
Lemiere Filip,
Dongen Walter Van,
Langridge James I.,
ElSharkawi Salah,
Games David E.,
Esmans Eddy L.,
Newton Russell P.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.428
Subject(s) - chemistry , chromatography , mass spectrometry , urinary system , high performance liquid chromatography , analytical chemistry (journal) , medicine , endocrinology
Qualitative and quantitative analyses of urinary nucleosides have diagnostic potential as tumour markers. We have developed separation techniques linked to mass spectrometric detection in order to overcome the problems associated with past identification and quantitation methods. The three methods of analysis utilised were: gas chromatography/mass spectrometry (GC/MS), high‐performance liquid chromatography/ion‐trap mass spectrometry (HPLC/ITMS) and capillary liquid chromatography/triple quadrupole mass spectrometry (CapLC/TQMS). Here we compare the relative effectiveness of each of the techniques for subsequent application in the systematic study of urinary nucleoside profiles in cancer patients. All three methods proved to be valuable techniques for such urinary nucleoside analyses, and a combination rather than one single choice is concluded as the ideal. Copyright © 2001 John Wiley & Sons, Ltd.