Premium
Optimization of quartz tube pyrolysis atmospheric pressure ionization mass spectrometry for the generation of bacterial biomarkers
Author(s) -
Tripathi Ashish,
Maswadeh Waleed M.,
Snyder A. Peter
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.427
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , pyrolysis , ionization , pyrolytic carbon , biomolecule , diketopiperazines , atmospheric pressure , chromatography , dipicolinic acid , ion , spore , organic chemistry , biochemistry , oceanography , geology , botany , biology
Experimental procedures were investigated to improve the efflux of biomolecule pyrolyzates from quartz tube pyrolysis under atmospheric pressure ionization mass spectrometry conditions. Heating regimes, airflows, and ion focusing parameters were optimized to increase the informative mass spectral signals generated from the pyrolysis of Gram‐positive bacterial spores and vegetative cells. Dipicolinic acid (DPA) is found in 5–15% by weight in Gram‐positive Bacillus spores, and the parameter optimization procedures provided an intense mass spectral signature of the m/z 168 protonated DPA molecule with a minimization of pyrolytic and ionization fragments. Moreover, mass spectral information from the optimization protocols yielded peaks and mass patterns characteristic of DNA and RNA nitrogen bases, protein diketopiperazines, and amino sugars. Copyright © 2001 John Wiley & Sons, Ltd.