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Mass spectrometry of intact neutral macromolecules using intense non‐resonant femtosecond laser vaporization with electrospray post‐ionization
Author(s) -
Brady John J.,
Judge Elizabeth J.,
Levis Robert J.
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4226
Subject(s) - chemistry , matrix assisted laser desorption electrospray ionization , mass spectrometry , femtosecond , desorption , analytical chemistry (journal) , laser , ionization , soft laser desorption , electrospray ionization , matrix assisted laser desorption/ionization , macromolecule , vaporization , adsorption , chromatography , ion , optics , organic chemistry , biochemistry , physics
Intact, nonvolatile, biological macromolecules can be transferred directly from the solid state into the gas phase, in ambient air, for subsequent mass spectral analysis using non‐resonant femtosecond (fs) laser desorption combined with electrospray ionization (ESI). Mass spectral measurements for neat samples, including a dipeptide, protoporphyrin IX and vitamin B12 adsorbed on a glass insulating surface, were obtained using an 800 nm, 70 fs laser with an intensity of 10 13 W cm −2 . No appreciable signal was detected when atmospheric matrix‐assisted or neat (matrix‐free) fs laser desorption was performed without ESI, indicating neutral desorption. Copyright © 2009 John Wiley & Sons, Ltd.