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Fast analysis of isobaric grape anthocyanins by Chip‐liquid chromatography/mass spectrometry
Author(s) -
Flamini Riccardo,
De Rosso Mirko,
Smaniotto Anna,
Panighel Annarita,
Vedova Antonio Dalla,
Seraglia Roberta,
Traldi Pietro
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4199
Subject(s) - chemistry , chromatography , mass spectrometry , isobaric process , anthocyanin , tandem mass spectrometry , electrospray , analytical chemistry (journal) , physics , food science , thermodynamics
In a previous work, direct‐infusion electrospray ionization ion trap tandem mass spectrometry (ESI‐IT‐MS/MS) was applied to the study of anthocyanins in extracts from the skins of Clinton grapes, a non‐ Vitis vinifera red grape variety qualitatively and quantitatively rich in anthocyanins. A good characterization of anthocyaninins was obtained, but it was impossible to differentiate some compounds with the same nominal mass but with different elemental composition. In this work, the capabilities of quadrupole time‐of‐flight mass spectrometry (QTOF‐MS) coupled with Chip‐liquid chromatography (LC‐Chip) were applied to the study of Clinton anthocyanins and this method provided the complete sample anthocyanin fingerprint in less than 5 min. Multi‐stage mass spectrometry (MS n ; n >2) was not necessary to identify isobaric compounds, nor were deuterium‐exchange experiments necessary to distinguish between compounds containing the same aglycone. The fast separation bypasses the problem of petunidin‐3‐ O ‐(6‐ O ‐acetyl)monoglucoside and delphinidin‐3,5‐ O ‐diglucoside quantification, present in the direct‐infusion ESI‐ITMS approach, due to overlapping with matrix interferences. Copyright © 2009 John Wiley & Sons, Ltd.