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Comparison of inert supports in laser desorption/ionization mass spectrometry of peptides: pencil lead, porous silica gel, DIOS‐chip and NALDI™ target
Author(s) -
Shenar Nawar,
Cantel Sonia,
Martinez Jean,
Enjalbal Christine
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4158
Subject(s) - chemistry , porous silicon , mass spectrometry , silicon , desorption , ionization , inert , chromatography , oligomer , matrix assisted laser desorption/ionization , porosity , analytical chemistry (journal) , organic chemistry , adsorption , ion
In the search for alternative inert surfaces replacing silicon chips in Desorption/Ionization On porous Silicon (DIOS)‐like mass spectrometry analyses, nanostructured silicon‐based NALDI™ chips were evaluated in Laser Desorption/Ionization (LDI) of peptides. Comparisons were made using commercially available DIOS chips (MassPREP™‐DIOS‐target™), amorphous carbon powder from lead pencil and porous silica gel used for chromatographic purposes as reference supports. A set of synthetic model peptides presenting variable amino acid sequences of various lengths was analyzed under all conditions. The LDI responses of the four ‘matrix‐free’ techniques were compared, especially in terms of peptide detection sensitivity and overall experiment robustness. Copyright © 2009 John Wiley & Sons, Ltd.