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Rapid identification of gallotannins from Chinese galls by matrix‐assisted laser desorption/ionization time‐of‐flight quadrupole ion trap mass spectrometry
Author(s) -
Zhu Fan,
Cai YiZhong,
Xing Jie,
Ke Jinxia,
Zhan Zhaoqi,
Corke Harold
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.4064
Subject(s) - chemistry , quadrupole ion trap , mass spectrometry , chromatography , quadrupole time of flight , fragmentation (computing) , ion trap , mass spectrum , analytical chemistry (journal) , electrospray ionization , operating system , computer science
Chinese gall, a conventional traditional Chinese medicine, contains high levels of gallotannins. A rapid method for direct analysis of the gallotannins without using any troublesome sample pretreatments was developed using matrix‐assisted laser desorption/ionization time‐of‐flight quadrupole ion trap mass spectrometry (MALDI‐QIT‐TOF MS) to successfully identify the gallotannin components in the crude extract of Chinese galls within several minutes. The high quality of the MS and MS 2 spectra acquired clearly showed that hydrolysable tannins in Chinese galls were identified as a series of the gallotannins with degrees of polymerization (DP) of 4–11 galloyl units. The MS 2 data indicated that the identified gallotannins with DP of 4–7 galloyl units had clear fragmentation with loss of 1–5 galloyl units which were further deprived of 1–3 water moieties. This technique may be used for rapid evaluation and screening of hydrolysable tannins in medicinal plants. Copyright © 2009 John Wiley & Sons, Ltd.