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Characterisation of anthocyanidins by electrospray ionisation and collision‐induced dissociation tandem mass spectrometry
Author(s) -
Oliveira M. C.,
Esperança P.,
Almoster Ferreira M. A.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.400
Subject(s) - chemistry , anthocyanidins , tandem mass spectrometry , collision induced dissociation , fragmentation (computing) , mass spectrometry , aglycone , electrospray ionization , dissociation (chemistry) , ion , analytical chemistry (journal) , computational chemistry , glycoside , stereochemistry , chromatography , organic chemistry , antioxidant , operating system , flavonoid , computer science
The present study describes the use of electrospray ionisation mass spectrometry, in combination with collision‐induced dissociation (CID) and tandem mass spectrometry, for the structural characterisation of anthocyanidins and their O ‐glycosides. The high‐energy CID spectra of [M−Cl] + ions of the free aglycones show characteristic fragmentation pathways, which provide useful information about the substitution pattern in the A‐ and B‐rings of each compound. The major fragmentation observed in the high‐energy CID spectra of [M−Cl] + ions of anthocyanins involves loss of the mono‐ or disaccharide units resulting in ions containing only the aglycone moiety. From the spectral data, the identity of the aglycone can be established as well as the number and the class of monosaccharide units in the O ‐glycosides. Copyright © 2001 John Wiley & Sons, Ltd.