Premium
Study of bisphosphonates by matrix‐assisted laser desorption/ionization mass spectrometry – influence of alkali atoms on fragmentation patterns
Author(s) -
Guénin Erwann,
Lecouvey Marc,
Hardouin Julie
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3984
Subject(s) - chemistry , fragmentation (computing) , mass spectrometry , alkali metal , ionization , desorption , electrospray ionization , protonation , ion , mass spectrum , analytical chemistry (journal) , ion source , sample preparation in mass spectrometry , chromatography , organic chemistry , adsorption , computer science , operating system
1‐Hydroxymethylene‐1,1‐bisphosphonic acids (or bisphosphonates) are compounds that have interesting pharmacological applications. However, few mass spectrometric investigations have been carried out to determine their fragmentation patterns. Herein, we evaluated different matrices for the study by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) of the formation and fragmentation of the protonated, the cationized (MNa + and MK + ) and the deprotonated bisphosphonates. Some in‐source fragmentations were observed both in positive and in negative ion modes. The fragmentation patterns obtained in post‐source decay mode are also discussed. In contrast to previous electrospray ionization/multi‐stage mass spectrometry (ESI‐MS n ) studies, some new fragmentation pathways were deduced and the effects of alkali ions on the fragmentation patterns were shown. The results summarized here completed the data previously recorded by ESI‐MS n and could be used for the characterization of bisphosphonates as alkali complexes in biological mixtures. Copyright © 2009 John Wiley & Sons, Ltd.