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N‐in‐one determination of retention factors for drugs by immobilized artificial membrane chromatography coupled to atmospheric pressure chemical ionization mass spectrometry
Author(s) -
Kangas Heli,
Kotiaho Tapio,
Salminen Timo,
Kostiainen Risto
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.398
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , mass spectrometry , acetonitrile , ammonium acetate , analytical chemistry (journal) , elution , chemical ionization , ionization , high performance liquid chromatography , ion , organic chemistry
Abstract Immobilized artificial membrane (IAM) chromatography is widely used in drug discovery for ranking the absorption properties of drug candidates. In this work an IAM chromatography method using atmospheric pressure chemical ionization mass spectrometric detection (IAM/APCI‐MS) was developed for the determination of log k IAM values for a mixture of compounds (9‐in‐one). Values were calculated from isocratic runs (0, 10, 20, 30, 35% acetonitrile) in both positive and negative modes. Good correlation (r 2 = 0.97) was achieved for n‐in‐one results obtained with ammonium acetate buffer and mass spectrometry, compared with the traditional method involving single compound analysis with phosphate buffered saline and an ultraviolet detector. A gradient elution method providing fast determination of relative log k IAM values in a single IAM/APCI‐MS run was demonstrated for the same compounds. Copyright © 2001 John Wiley & Sons, Ltd.