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Inter‐laboratory comparison of elemental analysis and gas chromatography/combustion/isotope ratio mass spectrometry. II. δ 15 N measurements of selected compounds for the development of an isotopic Grob test
Author(s) -
Calderone Giovanni,
Serra Francesca,
Lees Michele,
Mosandl Armin,
Reniero Fabiano,
Guillou Claude,
MorenoRojas José Manuel
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3956
Subject(s) - chemistry , mass spectrometry , isotope , isotope analysis , isotope ratio mass spectrometry , chromatography , radiochemistry , gas chromatography , elemental analysis , analytical chemistry (journal) , inorganic chemistry , nuclear physics , ecology , biology , physics
An inter‐laboratory exercise was carried out by a consortium of five European laboratories to establish a set of compounds, suitable for calibrating gas chromatography/combustion/isotope ratio mass spectrometry (GC‐C‐IRMS) devices, to be used as isotopic reference materials for hydrogen, carbon, nitrogen and oxygen stable isotope measurements. The set of compounds was chosen with the aim of developing a mixture of reference materials to be used in analytical protocols to check for food and beverage authentication. The exercise was organized in several steps to achieve the certification level: the first step consisted of the a priori selection of chemical compounds on the basis of the scientific literature and successive GC tests to set the analytical conditions for each single compound and the mixture. After elimination of the compounds that turned out to be unsuitable in a multi‐compound mixture, some additional oxygen‐ and nitrogen‐containing substances were added to complete the range of calibration isotopes. The results of δ 13 C determinations for the entire set of reference compounds have previously been published, while the δ D and δ 18 O determinations were unsuccessful and after statistical analysis of the data the results did not reach the level required for certification. In the present paper we present the results of an inter‐laboratory exercise to identify and test the set of nitrogen‐containing compounds present in the mixture developed for use as reference materials for the validation of GC‐C‐IRMS analyses in individual laboratories. Copyright © 2009 John Wiley & Sons, Ltd.