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Cell proteomic footprint
Author(s) -
Lokhov Petr,
Balashova Elena,
Dashtiev Maxim
Publication year - 2009
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3928
Subject(s) - chemistry , cell , footprint , authentication (law) , cell culture , computational biology , protease , throughput , microbiology and biotechnology , biological system , nanotechnology , biochemistry , computer science , biology , genetics , paleontology , telecommunications , computer security , wireless , enzyme , materials science
The authentication of mammalian cell cultures and their subpopulations is of great demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease under soft, ‘non‐killing’ conditions release fragments of cell surface proteins, whose composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of known cells. As an example, we applied this technique to verify subpopulations of human fibroblasts with different origins and which exhibit different medical characteristics. Copyright © 2009 John Wiley & Sons, Ltd.