Premium
5‐Ethyl‐2‐mercaptothiazole as matrix for matrix‐assisted laser desorption/ionization of a broad spectrum of analytes in positive and negative ion mode
Author(s) -
Raju N. Prasada,
Mirza Shama P.,
Vairamani M.,
Ramulu A. Raghu,
Pardhasaradhi M.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.392
Subject(s) - chemistry , analyte , matrix assisted laser desorption/ionization , matrix (chemical analysis) , mass spectrum , ionization , desorption , ion , analytical chemistry (journal) , matrix assisted laser desorption electrospray ionization , mass spectrometry , chromatography , ethyl lactate , organic chemistry , electron ionization , adsorption , catalysis
A preliminary investigation of the use of 5‐ethyl‐2‐mercaptothiazole as matrix in matrix‐assisted laser desorption/ionization (MALDI) of a broad spectrum of analytes is reported. The analytes studied are substance P, insulin, β‐cyclodextrin, triacylglycerols of coconut oil and polypropylene glycol 2000 (PPG 2000). In the positive ion mass spectra of the matrix/analyte combinations, the formation of [M + H] + and [M + cation] + species were observed and compared with those obtained by using well‐established matrices such as α‐cyano‐4‐hydroxycinnamic acid, genticic acid, sinapinic acid and dithranol. In addition, the usefulness of this new matrix for MALDI in negative ion mode is also described using substance P and β‐cyclodextrin as examples. Copyright © 2001 John Wiley & Sons, Ltd.