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Using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry to rapidly screen for albuminuria
Author(s) -
Shiea Jentaie,
Cho YiTzu,
Lin YenHsiu,
Chang ChinWen,
Lo LiHua,
Lee YiChern,
Ke HungLung,
Wu WenJeng,
Wu DengChyang
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3792
Subject(s) - chemistry , mass spectrometry , matrix assisted laser desorption/ionization , chromatography , albuminuria , detection limit , ionization , desorption , surface enhanced laser desorption/ionization , mass spectrum , matrix (chemical analysis) , analytical chemistry (journal) , sample preparation , ion , protein mass spectrometry , tandem mass spectrometry , organic chemistry , biochemistry , adsorption , renal function
Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices – sinapinic acid (SA), 2,5‐dihydroxybenzoic acid (2,5‐DHB), or 4‐hydroxy‐ α ‐cyanocinnamic acid ( α ‐CHC). Using this analytical approach, the limit of detection for albumin in urine is 10 −6 M, approximately 5 to 10 times lower than that detectable through conventional chemical testing. Copyright © 2008 John Wiley & Sons, Ltd.