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On‐line preconcentration and quantitative analysis of peptide hormone of brain and intestine using on‐column transient isotachophoresis coupled with capillary electrophoresis/electrospray ionization mass spectrometry
Author(s) -
Xia Shifei,
Zhang Lan,
Qiu Bin,
Lu Minghua,
Chi Yuwu,
Chen Guonan
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3787
Subject(s) - isotachophoresis , chemistry , chromatography , capillary electrophoresis , electrospray ionization , capillary electrophoresis–mass spectrometry , electrospray , mass spectrometry , electrolyte , capillary action , analytical chemistry (journal) , ion suppression in liquid chromatography–mass spectrometry , tandem mass spectrometry , electrode , composite material , materials science
A new approach was described to achieve very sensitive analysis of peptide hormone of brain and intestine by capillary electrophoresis coupled with a transient isotachophoresis (tITP) preconcentration method. The system used was electrospray ionization mass spectrometry (ESI‐MS) as detector and equipped with a sheath flow configuration. The effects of sample matrix, pH and concentration of leading electrolyte (LE), sample injection time, and ESI‐MS instrumental parameters on the efficiency of the sample stacking were investigated in detail. Under the optimized conditions, lower than micromole (0.01 µM) concentrations of the peptides were easily detected. Compared to traditional hydrodynamic injection methods, about 40–230‐fold increase in detection sensitivity was obtained by this technique. A distinguishing feature of the described approach is that the background electrolyte can serve as terminating electrolyte (TE), which simplifies the process of the experiments. The method was further evaluated by the analysis of low concentration active peptide mixtures spiked in hypothalamus tissue of the rat. Copyright © 2008 John Wiley & Sons, Ltd.