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Simultaneous determination and confirmation of melamine and cyanuric acid in animal feed by zwitterionic hydrophilic interaction chromatography and tandem mass spectrometry
Author(s) -
Heller David N.,
Nochetto Cristina B.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3779
Subject(s) - cyanuric acid , chemistry , melamine , chromatography , hydrophilic interaction chromatography , derivatization , mass spectrometry , tandem mass spectrometry , aqueous solution , liquid chromatography–mass spectrometry , analyte , high performance liquid chromatography , organic chemistry
We developed a new method to analyze animal feed and feed ingredients for melamine and cyanuric acid. The method is capable of extracting and detecting both melamine and cyanuric acid in a single procedure, whether present as free compounds or bound together as the melamine:cyanurate complex. A novel chromatographic system based on zwitterionic hydrophilic interaction chromatography (ZIC‐HILIC) columns enables separation and detection of both compounds in one run. Samples are extracted with a strong aqueous acid which is then diluted to bring the concentration within the working range of the method. The method is applicable over the range of 0.5 to 50 micrograms/gram (µg/g). Samples at higher concentrations may be diluted into this range, which is equivalent to 3.6–360 ng/mL in the injection solvent. Analytes are detected using liquid chromatography/tandem mass spectrometry. The data confirm the presence of both compounds according to criteria recommended by the US FDA Center for Veterinary Medicine. The LC/MS/MS method provides an alternative to derivatization and gas chromatography‐mass spectrometry for regulatory analysis of feed samples. Published in 2008 by John Wiley & Sons, Ltd.