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Stable isotope ratio analysis as a tool to discriminate between rainbow trout ( O. mykiss ) fed diets based on plant or fish‐meal proteins
Author(s) -
MorenoRojas J. M.,
Tulli F.,
Messina M.,
Tibaldi E.,
Guillou C.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3775
Subject(s) - rainbow trout , fish meal , corn gluten meal , chemistry , plant protein , meal , food science , meat and bone meal , zoology , feed conversion ratio , stable isotope ratio , fish <actinopterygii> , body weight , fishery , biology , endocrinology , physics , quantum mechanics
The use of stable isotope ratio analysis (SIRA) as a rapid analytical tool to characterize and discriminate farmed fish on the basis of the feedstuffs included in the diet formulation is discussed. Two isoproteic (44.8%) and isolipidic (19.6%) extruded diets were formulated: a fish‐meal‐based diet (FM diet), containing fish meal as the sole protein source; a plant‐protein‐based diet (PP diet), where pea protein concentrate and wheat gluten meal replaced 80% of fish meal protein. The diets were fed to eight groups of rainbow trout (initial body weight: 106.6g) for 103 days in two daily meals under controlled rearing conditions. Growth performance (final body weight: 318.5 g; specific growth rate: 1.06%) and feed‐to‐gain ratio (0.79) were not affected by the dietary treatment. The differences in isotopic values of the two diets were clearly reflected in the different carbon and nitrogen isotopic values in rainbow trout fillets. The δ 13 C and δ 15 N values of muscle of farmed rainbow trout showed differences between farmed fish fed a fish‐protein‐based diet (−20.47 ± 0.34 and 12.38 ± 0.57 for δ 13 C and δ 15 N, respectively) and those fed a plant‐protein‐based diet (−23.96 ± 0.38 and 7.15 ± 0.51 for δ 13 C and δ 15 N, respectively). The results suggest that SIRA provides a robust and verifiable analytical tool to discriminate between fish fed on a plant or a fish protein diet. Copyright © 2008 John Wiley & Sons, Ltd.