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High‐resolution liquid chromatography/electrospray ionization time‐of‐flight mass spectrometry combined with liquid chromatography/electrospray ionization tandem mass spectrometry to identify polyphenols from grape antioxidant dietary fiber
Author(s) -
Touriño Sonia,
Fuguet Elisabet,
Jáuregui Olga,
SauraCalixto Fulgencio,
Cascante Marta,
Torres Josep Lluís
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3756
Subject(s) - chemistry , chromatography , electrospray ionization , polyphenol , mass spectrometry , liquid chromatography–mass spectrometry , electrospray , extractive electrospray ionization , tandem mass spectrometry , sample preparation in mass spectrometry , direct electron ionization liquid chromatography–mass spectrometry interface , protein mass spectrometry , ionization , antioxidant , chemical ionization , ion , organic chemistry
Abstract Grape antioxidant dietary fiber (GADF) is a dietary supplement that combines the benefits of both fiber and antioxidants that help prevent cancer and cardiovascular diseases. The antioxidant polyphenolic components in GADF probably help prevent cancer in the digestive tract, where they are bioavailable. Mass spectrometry coupled to liquid chromatography is a powerful tool for the analysis of complex plant derivatives such as GADF. We use a combination of MS techniques, namely liquid chromatography/electrospray ionization time‐of‐flight mass spectrometry (LC/ESI‐TOF‐MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) on a triple quadrupole, for the identification of the polyphenolic constituents of the soluble fraction of GADF. First, we separated the mixture into four fractions which were tested for phenolic constituents using the TOF system in the full scan mode. The high sensitivity and resolution of the TOF detector over the triple quadrupole facilitate the preliminary characterization of the fractions. Then we used LC/ESI‐MS/MS to identify the individual phenols through MS/MS experiments (product ion scan, neutral loss scan, precursor ion scan). Finally, most of the identities were unequivocally confirmed by accurate mass measurements on the TOF spectrometer. LC/ESI‐TOF‐MS combined with MS/MS correctly identifies the bioactive polyphenolic components from the soluble fraction of GADF. High‐resolution TOF‐MS is particularly useful for identifying the structure of compounds with the same LC/ESI‐MS/MS fragmentation patterns. Copyright © 2008 John Wiley & Sons, Ltd.