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Simultaneous analysis of thirteen diuretics residues in bovine milk by ultra‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Shao Bing,
Zhang Jing,
Yang Yi,
Meng Juan,
Wu Yongning,
Duan Hejun
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3740
Subject(s) - chemistry , chromatography , analyte , tandem mass spectrometry , electrospray ionization , mass spectrometry , liquid chromatography–mass spectrometry , ion suppression in liquid chromatography–mass spectrometry , selected reaction monitoring , hexane
A simple, rapid, sensitive and specific method used to screen and confirm multi‐class diuretics residues in whole bovine milk is described. Thirteen drugs of four different classes including carbonic anhydrase inhibitors, loop, thiazide and potassium‐sparing diuretics were extracted from whole milk by acetonitrile followed by further purification with hexane. The analytes were separated using an ACQUITY UPLC™ BEH C18 column and detected by electrospray ionization tandem mass spectrometry (ESI‐MS/MS). MS data acquisition was performed by a time‐scheduled multiple reaction monitoring program, selecting two ion transitions for each target compound. The overall average recoveries based on matrix‐fortified curves fortified with diuretics at three levels ranged from 80.6 to 108.8% with the coefficients of variation ranging from 2.6 to 19.7% ( n = 6). The limits of quantitation (LOQs) of diuretics in bovine milk were 5.0 µg/kg for spironolactone and 0.5 µg/kg for other analytes, respectively. Copyright © 2008 John Wiley & Sons, Ltd.