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Real‐time, on‐line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry
Author(s) -
Zhu Liang,
Gamez Gerardo,
Chen Huan Wen,
Huang Hao Xi,
Chingin Konstantin,
Zenobi Renato
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3700
Subject(s) - chemistry , extractive electrospray ionization , mass spectrometry , tandem mass spectrometry , electrospray ionization , selected reaction monitoring , direct electron ionization liquid chromatography–mass spectrometry interface , chemical ionization , desorption electrospray ionization , chromatography , ionization , organic chemistry , ion
Extractive electrospray ionization mass spectrometry (EESI‐MS) for real‐time monitoring of organic chemical reactions was demonstrated for a well‐established pharmaceutical process reaction and a widely used acetylation reaction in the presence of a nucleophilic catalyst, 4‐dimethylaminopyridine (4‐DMAP). EESI‐MS provides real‐time information that allows us to determine the optimum time for terminating the reaction based on the relative intensities of the precursors and products. In addition, tandem mass spectrometric (MS/MS) analysis via EESI‐MS permits on‐line validation of proposed reaction intermediates. The simplicity and rapid response of EESI‐MS make it a valuable technique for on‐line characterization and full control of chemical and pharmaceutical reactions, resulting in maximized product yield and minimized environmental costs. Copyright © 2008 John Wiley & Sons, Ltd.