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Carbon isotope effects of DDTs in carrot during the digestion process using an in vitro test
Author(s) -
Yu Yingxin,
Lu Min,
Jia Wanglu,
Van de Wiele Tom,
Han Shuyuan,
Zhang Dongping,
Wu Minghong,
Sheng Guoying,
Fu Jiamo
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3682
Subject(s) - chemistry , digestion (alchemy) , isotope , isotopes of carbon , environmental chemistry , digestive tract , isotope analysis , radiochemistry , chromatography , total organic carbon , medicine , ecology , biology , physics , quantum mechanics
Compound‐specific carbon isotope ratio analysis is a promising tool to assess the origins and fates of organic contaminants in many fields. The present study aims to investigate the isotope effects of dichloro‐diphenyl‐trichloroethane (DDT) and its degradates in carrots during digestion processes simulating the human gastrointestinal tract. To accurately interpret isotopic data obtained for unreleased DDTs ( p,p ‐DDT, p,p ‐DDD and p,p ‐DDE) from carrots during digestion, spiked carrots with known δ 13 C values of DDTs were incubated in simulated gastric and intestinal solutions using a static in vitro model of the human gut. The δ 13 C values and concentrations of DDTs remaining in the carrot and in the digestive solutions were measured. The difference in the δ 13 C values of the added DDTs and the matrix‐bound DDTs after digestion was always <0.50‰, which is within the technical specification of the analytical system, although the released DDTs increased or decreased under the digestion conditions. The study demonstrated that there were no significant carbon isotopic fractionations during digestion. To our knowledge, this is the first report demonstrating the isotope effect of organic compounds during stomach and small intestine digestion, and the first reported isotope analyses of DDTs. Copyright © 2008 John Wiley & Sons, Ltd.