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Rapid and sensitive quantitation of antibiotics in fermentations by electrospray mass spectrometry
Author(s) -
Morgan Janine,
JoyceMenekse Miranda E.,
Rowlands Robert T.,
Gilbert Ian H.,
Lloyd David
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.368
Subject(s) - chemistry , chromatography , electrospray , mass spectrometry , electrospray ionization , antibiotics , biochemistry
Electrospray ionisation mass spectrometry (ESI‐MS) has been used for the determination and quantitation of a broad range of 24 antibiotics, from groups including aminoglycosides, β‐lactams, tetracyclines, antifungals and glycopeptides. Spectra have been acquired for all 24 antibiotics derived from pure samples dissolved in acetonitrile/water, along with samples extracted from complex fermentation liquor. Quantitation was carried out by the detection of the protonated molecules, using time‐scheduled single‐ion monitoring (SIM). ESI‐MS was used to detect and quantify to 5‐µM levels. A one‐step extraction of antibiotics with an organic solvent (methanol) was used for this rapid and simple procedure. Specificity is not matched by other methods and antibiotic analogues (e.g. the five forms of erythromycin) can be determined within minutes. Copyright © 2001 John Wiley & Sons, Ltd.