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Improved imaging resolution in desorption electrospray ionization mass spectrometry
Author(s) -
Kertesz Vilmos,
Van Berkel Gary J.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3662
Subject(s) - desorption electrospray ionization , chemistry , mass spectrometry , resolution (logic) , extractive electrospray ionization , analyte , analytical chemistry (journal) , desorption , electrospray ionization , ionization , electrospray , ambient ionization , maldi imaging , image resolution , chromatography , sample preparation in mass spectrometry , matrix assisted laser desorption/ionization , optics , chemical ionization , adsorption , ion , artificial intelligence , physics , organic chemistry , computer science
The imaging resolution of desorption electrospray ionization mass spectrometry (DESI‐MS) was investigated using printed patterns on paper and thin‐layer chromatography (TLC) plate surfaces. Resolution approaching 40 µm was achieved with a typical DESI‐MS setup, which is approximately 5 times better than the best resolution reported previously. This improvement was accomplished with careful control of operational parameters (particularly spray tip‐to‐surface distance, solvent flow rate, and spacing of lane scans). In addition, an appropriately strong analyte/surface interaction and uniform surface texture on the size scale no larger than the desired imaging resolution were required to achieve this resolution. Overall, conditions providing the smallest possible effective desorption/ionization area in the DESI impact plume region and minimizing the analyte redistribution on the surface during analysis led to improved DESI‐MS imaging resolution. Published in 2008 by John Wiley & Sons, Ltd.