Premium
Identification of the major metabolites of tectorigenin in rat bile by liquid chromatography combined with time‐of‐flight and ion trap tandem mass spectrometry
Author(s) -
Zhang WeiDong,
Qi LianWen,
Yang XiaoLin,
Huang WenZhe,
Li Ping,
Yang ZhongLin
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3660
Subject(s) - chemistry , chromatography , mass spectrometry , time of flight mass spectrometry , liquid chromatography–mass spectrometry , ion trap , tandem mass spectrometry , ion , organic chemistry , ionization
A novel methodology for the identification of tetorigenin and its metabolites in rat bile has been created using liquid chromatography (LC) combined with time‐of‐flight (TOF) and ion trap multiple mass spectrometry (IT‐MS n ). As a means to discriminate amongst unknown organic compounds in complex biological matrices, the proposed methodology relies upon the combination of LC/TOF‐MS to provide accurate mass measurements in generating a molecular formula while benefiting from the complementary structural information provided by the LC/IT‐MS n . In this study, the combined approach has been applied to the metabolic fingerprinting chromatograms of rat bile samples before and after tectorigenin administration. All possible metabolites are investigated based on accurate mass data and isotope function using LC/TOF‐MS and structural confirmation using LC/IT‐MS n . Seven phase II metabolities of tectorigenin in rat bile have been successfully elucidated using this novel LC approach and are being reported for the first time. Copyright © 2008 John Wiley & Sons, Ltd.