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Discrimination of Penicillium isolates by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry fingerprinting
Author(s) -
Hettick Justin M.,
Green Brett J.,
Buskirk Amanda D.,
Kashon Michael L.,
Slaven James E.,
Janotka Erika,
Blachere Francoise M.,
Schmechel Detlef,
Beezhold Donald H.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3649
Subject(s) - chemistry , mass spectrometry , chromatography , penicillium , mass spectrum , analytical chemistry (journal) , matrix assisted laser desorption/ionization , time of flight mass spectrometry , trifluoroacetic acid , ionization , desorption , organic chemistry , ion , food science , adsorption
Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) was used to generate highly reproducible mass spectral ‘fingerprints’ for twelve Penicillium species. Prior to MALDI‐TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one‐minute bead‐beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5000–20 000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13 900. Discriminant analysis using the MALDI‐TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI‐TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance. Published in 2008 by John Wiley & Sons, Ltd.