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Identification and quantitation of auxins in plants by liquid chromatography/electrospray ionization ion trap mass spectrometry
Author(s) -
Lu Qiaomei,
Zhang Lan,
Chen Tianwen,
Lu Minghua,
Ping Tong,
Chen Guonan
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3642
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray ionization , extractive electrospray ionization , ion trap , direct electron ionization liquid chromatography–mass spectrometry interface , sample preparation in mass spectrometry , electrospray , top down proteomics , protein mass spectrometry , ion , chemical ionization , ionization , organic chemistry
Auxin is an important phylohormone, which regulates specific physiological responses such as division, elongation and differentiation of cells. A new method using liquid chromatography/electrospray ionization ion trap mass spectrometry (LC/ESI‐ITMS) has been developed for identification and quantitation of four auxins. Under the optimum conditions, four auxins (indole‐3‐acetic acid, indole‐3‐propionic acid, indole‐3‐butyric acid and 1‐naphthylacetic acid) were completely separated and quantitated within 7 min with a minimum detection limit of 8.0 ng mL −1 with relative standard deviations lower than 5.0%. This method also has been applied to analysis of auxins in Chinese cabbage where, even with a complicated serious background perturbation due to the natural biological matrix, the mean recoveries ranged from 77.5% to 99.8%. Finally, we discuss the MS‐relevant properties of the identified auxins in detail. Copyright © 2008 John Wiley & Sons, Ltd.