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Coupling of a supercritical fluid chromatography system to a hybrid (Q‐TOF 2) mass spectrometer: on‐line accurate mass measurements
Author(s) -
Garzotti Marco,
Rovatti Luca,
Hamdan Mahmoud
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.364
Subject(s) - chemistry , mass spectrometry , analyser , supercritical fluid chromatography , spectrometer , chromatography , detector , supercritical fluid , resolution (logic) , analytical chemistry (journal) , coupling (piping) , hybrid mass spectrometer , mass spectrum , mass fraction , high performance liquid chromatography , selected reaction monitoring , optics , tandem mass spectrometry , artificial intelligence , organic chemistry , physics , mechanical engineering , computer science , engineering
This note describes a simple and economical method to couple a supercritical fluid chromatography (SFC) system (Berger Instruments, US) with a high‐resolution hybrid mass spectrometer (Q‐TOF 2; Micromass, UK). This experimental arrangement has three distinct advantages: (1) coupling between the two systems can be effected without the need for an interface or hardware modifications of either system, (ii) this experimental arrangement provides on‐line accurate mass SFC/MS measurements which are indispensable for the characterisation of new chemical entities and unknown metabolites, and (iii) the characteristically fast spectral acquisition rate of the time‐of‐flight (TOF) analyser renders the present arrangement an important contribution to future semipreparative fraction collection setups which use mass spectrometry as a detector. Copyright © 2001 John Wiley & Sons, Ltd.