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New features on the fragmentation and differentiation of C ‐glycosidic flavone isomers by positive electrospray ionization and triple quadrupole mass spectrometry
Author(s) -
AbadGarcía Beatriz,
GarmónLobato Sergio,
Berrueta Luis A.,
Gallo Blanca,
Vicente Francisca
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3560
Subject(s) - chemistry , triple quadrupole mass spectrometer , fragmentation (computing) , electrospray ionization , mass spectrometry , tandem mass spectrometry , electrospray , chromatography , luteolin , glycoside , apigenin , analytical chemistry (journal) , selected reaction monitoring , flavonoid , stereochemistry , organic chemistry , computer science , antioxidant , operating system
Six flavone mono‐ C‐ glucosides, four standards (β‐D‐glucopyranosyl‐(1 → C‐6)‐ and ‐(1 → C‐8)‐ apigenin and luteolin) and two others from lemon juice (β‐D‐glucopyranosyl‐(1 → C‐6)‐ and ‐(1 → C‐8)‐diosmetin) have been studied in order to analyze their fragmentation patterns. Initial separation was carried out using high‐performance liquid chromatography with diode‐array detection (HPLC/DAD) coupled to an electrospray ionization (ESI) interface and a triple quadrupole mass spectrometer. Several systematic differences between collision‐induced dissociation tandem mass (CID‐MS/MS) spectra of C‐6‐ and C‐8‐isomers have been found and some general guidelines and two new diagnostic product ions have been proposed for the differentiation of C‐6‐ and C‐8‐flavonoid glycosides. These results have been successfully applied to the characterization of two flavone C ‐glycosides found in lemon juice, and mass spectra of a flavone di‐ C ‐glycoside detected in lemon juice have been studied and interpreted. Copyright © 2008 John Wiley & Sons, Ltd.