Premium
A comprehensive on‐line digestion‐liquid chromatography/mass spectrometry/collision‐induced dissociation mass spectrometry approach for the characterization of human fibrinogen
Author(s) -
Vecchione Gennaro,
Casetta Bruno,
Santacroce Rosa,
Margaglione Maurizio
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.355
Subject(s) - chemistry , mass spectrometry , chromatography , fibrinogen , dissociation (chemistry) , collision induced dissociation , digestion (alchemy) , sample preparation in mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , biochemistry , organic chemistry , electrospray ionization
An automatic on‐line digestion‐liquid chromatography/mass spectrometry/collision‐induced dissociation mass spectrometry (LC‐MS/CID‐MS) protocol has been developed for detection of errors in the biosynthesis of human fibrinogen, such as amino acid (AA) mismatch or incorrect post‐translational modification (PTM). Using on‐line digestion on an immobilized‐enzyme column, the reaction time is significantly reduced (less than 20 min) and the entire approach is suitable for automation. The two‐loop MS experiments (full‐scan acquisition and sugar moieties monitoring by SIM) allow checking both the correct AA mapping via the peptides generated by the digestion of the PTM. Since the protocol was designed for application on a routine basis, as a proof‐of‐concept detection of a rare case of ‘abnormal’ fibrinogen has been demonstrated. The advantage of the proposed approach is exemplified by the fact that the DNA sequence information for the case investigated had not shown any evidence of the abnormality. Copyright © 2001 John Wiley & Sons, Ltd.